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cyanine5 5 cy5 5  (MedChemExpress)


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    Structured Review

    MedChemExpress cyanine5 5 cy5 5
    Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of <t>Cy5.5-labeled</t> DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.
    Cyanine5 5 Cy5 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyanine5+5+cy5+5/Cyanine/pmc13054405-48-0-8
    Average 94 stars, based on 24 article reviews
    cyanine5 5 cy5 5 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis"

    Article Title: Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis

    Journal: International Journal of Pharmaceutics: X

    doi: 10.1016/j.ijpx.2026.100528

    Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of Cy5.5-labeled DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of Cy5.5-labeled DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.

    Techniques Used: In Vivo, Fluorescence, Injection, Labeling, Ex Vivo

    Related Articles

    Modification:

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin–streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), l -arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4′,6-diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin—streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), L-arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4 ′ ,6- diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.

    Lysis:

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin–streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), l -arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4′,6-diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin—streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), L-arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4 ′ ,6- diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.

    Cell Culture:

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin–streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), l -arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4′,6-diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.

    Article Title: Engineered Escherichia coli Nissle 1917 targeted delivery of extracellular PD-L1-mFc fragment for treating inflammatory bowel disease
    Article Snippet: .. Dulbecco’s modified Eagle’s medium with high glucose (DMEM) (Punosai Biotechnology Co., Ltd., FM50210, Wuhan, China), fetal bovine serum (Novizan Biotechnology Co., Ltd., F101-03, Nanjing, China), 100 × penicillin—streptomycin solution (Xinsaimei Biotechnology Co., Ltd., C100C5, Suzhou, China), L-arabinose (Aladdin Biotechnologies, A106195-100g, Shanghai, China), TieChui E. coli Lysis Buffer (Boyi Biological Technology Co., Ltd., BR0005-02/BR0005-03, Changzhou, China), 4 ′ ,6- diamidino-2-phenylindole (DAPI) (Sikejie Biotechnology Co., Ltd., EE0015, Shandong, China), cell culture plates (Sening Biotechnology Co., Ltd., 1022000, Hangzhou, China), 96-well plates (Sening Biotechnology Co., Ltd., 1014000), ampicillin (Target Molecule Corp., T0814L-200 mg, Boston, MA, USA), Cyanine5.5 (Cy5.5) (MedChem Express, HY-D0924, Shanghai, China), and rabbit polyclonal anti-FLAG tag antibody (Proteintech, 20543-1-AP, Wuhan, China). .. Environmentally friendly dewaxing transparent liquid (G1128), 20 × citric acid antigen retrieval solution (pH 6.0) (G1202), 20 × Tris-EDTA antigen retrieval solution (pH 9.0) (G1203), 20 × Tris-EDTA antigen retrieval solution (pH 8.0) (G1206), DAB chromogenic solution (G1212), and hematoxylin (G1004) were purchased from Wuhan Saiwei Biotechnology Co., Ltd.



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    Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of <t>Cy5.5-labeled</t> DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.
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    Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of Cy5.5-labeled DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.

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    Article Title: Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis

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    Figure Lengend Snippet: Biodistribution of M2-EVs@DNase I in septic mice. (A) Representative in vivo fluorescence images of septic mice following intravenous injection of Cy5.5-labeled DNase I or M2-EVs@DNase I at 3, 6, 12, and 24 h. (B) Ex vivo fluorescence images of major organs at 24 h post-administration. (C) Quantitative results of fluorescence intensity in major organs. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01.

    Article Snippet: Cyanine5.5 (Cy5.5) and DNase I were acquired from MedChemExpress (MCE, NJ, USA).

    Techniques: In Vivo, Fluorescence, Injection, Labeling, Ex Vivo

    In vitro activity of Fe/SAN. A: Confocal microscopy observation of the distribution of Cy5.5-Fe/SAN in HT22 cells at different time points (1–4 h). B: Colocalization analysis of Cy5.5-Fe/SAN with lysosomes. C: Effects of Fe/SAN on survival status of glutamate-injured HT22 cells. D: Effects of Fe/SAN on ROS levels in HT22 cells. E: Effects of Fe/SAN on mitochondrial membrane potential in HT22 cells. F: Effects of Fe/SAN on glutamate-induced apoptosis in HT22 cells. G: Effects of Fe/SAN on the expression of HT22 cell-related proteins (P65, P-P65, IKBα, Bax, and Bcl-2). H: Gray scale analysis of Western blot bands. I: Quantitative analysis of the intracellular relative fluorescence intensity changes over time. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Materials Today Bio

    Article Title: Iron single-atom nanozyme-mediated laser interstitial thermal therapy and anti-inflammatory effect for epilepsy

    doi: 10.1016/j.mtbio.2026.102810

    Figure Lengend Snippet: In vitro activity of Fe/SAN. A: Confocal microscopy observation of the distribution of Cy5.5-Fe/SAN in HT22 cells at different time points (1–4 h). B: Colocalization analysis of Cy5.5-Fe/SAN with lysosomes. C: Effects of Fe/SAN on survival status of glutamate-injured HT22 cells. D: Effects of Fe/SAN on ROS levels in HT22 cells. E: Effects of Fe/SAN on mitochondrial membrane potential in HT22 cells. F: Effects of Fe/SAN on glutamate-induced apoptosis in HT22 cells. G: Effects of Fe/SAN on the expression of HT22 cell-related proteins (P65, P-P65, IKBα, Bax, and Bcl-2). H: Gray scale analysis of Western blot bands. I: Quantitative analysis of the intracellular relative fluorescence intensity changes over time. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Cyanine5.5 NHS ester (Cy5.5) is from Lumiprobe (Hunt Valley, MD, USA).

    Techniques: In Vitro, Activity Assay, Confocal Microscopy, Membrane, Expressing, Western Blot, Fluorescence